Table 2. Oligonucleotides used for QuikChange mutagenesis
NoSequence motifPrimer sequence (5′→3′)
1aZnF1 H>A mutation forGCAAATATTATCTAAAAGTCGCCAACAGGAAACATACTGGGGAGAAACCC
1bZnF1 H>A mutation revTCCCCAGTATGTTTCCTGTTGGCGACTTTTAGATAATATTTGCTGAGGAA
2aZnF3 H>A mutation forGAAGGATGGAGCTGCGGGTGGCCATGGTGTCTCACACAGGGGAGATGCCC
2bZnF3 H>A mutation revTCCCCTGTGTGAGACACCATGGCCACCCGCAGCTCCATCCTTCGGCGGAA
3aZnF4 H>A mutation forAGAAGAAGGACTTGCAGAGCGCCATGATCAAACTTCATGGAGCCCCCAAG
3bZnF4 H>A mutation revGCTCCATGAAGTTTGATCATGGCGCTCTGCAAGTCCTTCTTCTGCATGAA
4aZnF5 H>A mutation forCTCGGACAGAGCTGCAGCTGGCTGAAGCTTTCAAGCACCGTGGTGAGAAG
4bZnF5 H>A mutation revCCACGGTGCTTGAAAGCTTCAGCCAGCTGCAGCTCTGTCCGAGACAGGAA
5aZnF6 H>A mutation forGCCGGAATGGCCTGCAGATGGCCATCAAGGCCAAGCACAGGAATGAGAGG
5bZnF6 H>A mutation revTTCCTGTGCTTGGCCTTGATGGCCATCTGCAGGCCATTCCGGCTCGAGGC
6aZnF7 H>A mutation forAAAAGGCCAATCTCAACATGGCCCTGCGCACACACACGGGTGAGAAGCCC
6bZnF7 H>A mutation revTCACCCGTGTGTGTGCGCAGGGCCATGTTGAGATTGGCCTTTTGGGTGAA
7aZnF8 H>A mutation forCCCAAGCCAGCCTGGACAAGGCCAACCGCACCCACACCGGGGAAAGGCCC
7bZnF8 H>A mutation revTCCCCGGTGTGGGTGCGGTTGGCCTTGTCCAGGCTGGCTTGGGTTCGGAA
8aZnF9 H>A mutation forAGAAGGGGCCCCTCCTGAGGGCCGTGGCCAGCCGCCATCAGGAGGGCCGG
8bZnF9 H>A mutation revTCCTGATGGCGGCTGGCCACGGCCCTCAGGAGGGGCCCCTTCTCAGTGAA
9aZnF10 H>A mutation forCCGTGGAGCAACTGCGTGTGGCCGTCAGACGGCACAAGGGGGTGAGGAAG
9bZnF10 H>A mutation revACCCCCTTGTGCCGTCTGACGGCCACACGCAGTTGCTCCACGGCTTTGAA
10aZnF11 H>A mutation forGACAGGCCCACCTGCGGAGGGCCATGGAGATCCACGACCGGGTAGAGAAC
10bZnF11 H>A mutation revACCCGGTCGTGGATCTCCATGGCCCTCCGCAGGTGGGCCTGTCGGGTAAA
11a∆ZnF 1‐3 mutation forGGGCATCTCCCCTGTCGGCACCGCTGTACCTTTTCTGTTC
11b∆ZnF 1‐3 mutation revGGTACAGCGGTGCCGACAGGGGAGATGCCCTACAAGTGTT
12a∆ZnF 4‐6 mutation forTGGCCTCTCATTCCTGGCATCTCCCCTGTGTGAGACACCA
12b∆ZnF 4‐6 mutation revCACAGGGGAGATGCCAGGAATGAGAGGCCACACGTATGTG
13a∆ZnF 7‐9 mutation forAGGAATGAGAGGCCACAGGAGGGCCGGCCCCACTTCTGCC
13b∆ZnF 7‐9 mutation revGGGCCGGCCCTCCTGTGGCCTCTCATTCCTGTGCTTGGCC
14a∆ZnF 7‐11 mutation forAGGAATGAGAGGCCAGACCGGGTAGAGAACTACAACCCGC
14b∆ZnF 7‐11 mutation revGTTCTCTACCCGGTCTGGCCTCTCATTCCTGTGCTTGGCC
15a∆ZnF 1‐9 mutation forGGTACAGCGGTGCCGCAGGAGGGCCGGCCCCACTTCTGCC
15b∆ZnF 1‐9 mutation revGGGCCGGCCCTCCTGCGGCACCGCTGTACCTTTTCTGTTC
16a∆ZnF 1‐10 mutation forGGTACAGCGGTGCCGAAGGGGGTGAGGAAGTTTGAGTGCA
16b∆ZnF 1‐10 mutation forCTTCCTCACCCCCTTCGGCACCGCTGTACCTTTTCTGTTC