Table 1. NMR and refinement statistics for the Rtt103p CID‐pThr4 CTD complex
Rtt103p CID–pThr4 CTD complex
NMR distance & dihedral constraints
Distance restraints
Total NOEs3,639
Intra‐residue843
Inter‐residue2,796
Short1,691
Medium1,104
Long844
Hydrogen bonds99
Intermolecular distance restraints47
Total dihedral angle restraintsa198
Structure statistics b
Violations (mean and s.d.)
Number of distance restraint violations > 0.5 Å0.10 ± 0.31
Number of dihedral angle restraint violations > 15°13.8 ± 2.33
Maximum dihedral angle restraint violation (°)39.57 ± 9.55
Maximum distance constraint violation (Å)0.30 ± 0.12
Deviations from idealized geometryb
Bond lengths (Å)0.00355 ± 0.00008
Bond angles (°)1.702 ± 0.012
Average pairwise r.m.s.d. (Å)b
Rtt103p CID (7–12; 19–31; 36–48; 54–73; 77–94; 100–116; 121–133)
Heavy atoms0.91 ± 0.13
Backbone atoms0.25 ± 0.05
CTD (143–152)
Heavy atoms2.06 ± 0.42
Backbone atoms1.42 ± 0.34
Complex
All complex heavy atoms1.12 ± 0.16
All complex backbone atoms0.61 ± 0.14
Ramachandran plot statisticsc
Residues in most favoured regions (%)88.5
Residues in additionally allowed regions (%)10.1
Residues in generously allowed regions (%)0.9
Residues in disallowed regions (%)0.5
  • a α‐helical dihedral angle restraints imposed for the backbone based on the CSI.

  • b Calculated for an ensemble of the 20 lowest energy structures.

  • c Based on PROCHECK analysis [42].