Table 1. Properties of the PRC1‐labeled overlap measured by different approaches
Tubulin‐GFP cells immunostained for PRC1PRC1‐GFP cells fixedPRC1‐GFP cells live
I/au1778.78 ± 101.37311.34 ± 16.49343.69 ± 30.23
Icross/au53.03 ± 4.859.56 ± 0.4415.01 ± 1.51
LPRC1/μm4.95 ± 0.185.49 ± 0.174.64 ± 0.08
Spindle length/μmND10.65 ± 0.16ND
Spindle width/μmND11.21 ± 0.25ND
  • Au, arbitrary units; ND, not determined.

  • All values are given as mean ± s.e.m. and measured as in Materials and Methods.