Table 2. Properties of different cell lines treated with siRNA
ControlPRC1 siRNA% of changeP‐value
Unlabeled cells
Western blot0.81 ± 0.080.40 ± 0.0151.10 ± 5.490.0092
Tubulin‐GFP‐labeled cells
Western blot1.25 ± 0.290.58 ± 0.0553.40 ± 11.760.0476
I b 13.16 ± 1.409.45 ± 1.4028.19 ± 13.090.0744
I bk 29.12 ± 2.7726.94 ± 3.587.48 ± 15.110.6503
Ik = IbkIb15.95 ± 1.4917.49 ± 2.308.80 ± 14.710.6042
I b /I bk 0.44 ± 0.010.33 ± 0.0125.00 ± 2.840.0003
dk/μm1.00 ± 0.020.88 ± 0.0212.00 ± 2.640.0001
Spindle length/μm10.79 ± 0.1011.06 ± 0.092.45 ± 1.260.0722
Spindle width/μm11.42 ± 0.2911.63 ± 0.431.81 ± 4.550.6835
Tubulin‐GFP‐labeled cells immunostained for PRC1
LPRC1/μm4.96 ± 0.08
I/au717.77 ± 15.27421.18 ± 8.5841.33 ± 1.700.0001
PRC1‐GFP‐labeled cells immunostained for PRC1
Western blot0.86 ± 0.040.41 ± 0.0451.78 ± 5.400.0020
LPRC1 immunostained/μm4.99 ± 0.064.91 ± 0.101.60 ± 2.540.51667
LPRC1‐GFP/μm5.36 ± 0.145.15 ± 0.163.91 ± 3.890.3407
Iimmunostained PRC1/au2550.80 ± 125.341918.98 ± 99.8925.77 ± 5.380.0003
I PRC1‐GFP/au1551.59 ± 83.97965.30 ± 51.5737.79 ± 4.730.0001
Icross immunostained/au54.06 ± 2.9935.43 ± 2.4834.46 ± 5.840.0001
Icross PRC1‐GFP/au29.92 ± 2.4216.66 ± 0.8144.31 ± 5.250.0001
Spindle length/μm10.81 ± 0.2310.59 ± 0.322.03 ± 3.570.5897
Spindle width/μm9.51 ± 0.339.20 ± 0.243.25 ± 4.160.4592
  • All values are given as mean ± s.e.m. and measured as described in the Materials and Methods section (au = arbitrary units).