Table 1. Quantification of RE dynamics in HeLa cells
Transfection of DNA/shRNAAvg. no. of tubules/cell (TN)Avg. length/tubule (TL in μm)
Control sh22.84 ± 2.303.03 ± 0.10
Rab4A sh13.96 ± 2.693.02 ± 0.13
Rab5A sh29.14 ± 3.973.01 ± 0.10
Rab5B sh16.13 ± 2.003.10 ± 0.13
Rab5C sh10.19 ± 2.512.43 ± 0.10
Rab7A sh2.70 ± 0.412.17 ± 0.17
Rab9A sh14.00 ± 2.312.63 ± 0.10
Rab11A sh4.05 ± 1.141.82 ± 0.08
Rab14A sh36.68 ± 4.823.36 ± 0.11
Rab22A sh2.54 ± 0.371.73 ± 0.09
Rab22AWT24.85 ± 1.302.92 ± 0.10
Rab22AQ64L26.86 ± 3.303.18 ± 0.14
Rab22AS19N1.35 ± 0.251.84 ± 0.25
Control sh24.48 ± 2.802.93 ± 0.08
Rab22A sh3.90 ± 0.462.27 ± 0.10
BLOC‐1 sh4.39 ± 0.482.53 ± 0.09
BLOC‐2 sh3.24 ± 0.322.19 ± 0.10
  • HeLa cells were transfected with respective shRNAs or Rab22A constructs as indicated. Cells were further transfected with KIF13A‐YFP, fixed with methanol and then imaged. Images were analysed for RE dynamics using Fiji Macro programme plug‐in as described in Materials and Methods. Data are derived from three independent experiments (= 3, mean ± SEM) and represented as graphs in Figs 1B and C or 2B and C or 3B and C.