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Formation of GW bodies is a consequence of microRNA genesis

Kaleb M Pauley, Theophany Eystathioy, Andrew Jakymiw, John C Hamel, Marvin J Fritzler, Edward K L Chan

Author Affiliations

  1. Kaleb M Pauley1,,
  2. Theophany Eystathioy1,2,,
  3. Andrew Jakymiw1,
  4. John C Hamel1,
  5. Marvin J Fritzler2 and
  6. Edward K L Chan*,1
  1. 1 Department of Oral Biology, University of Florida, 1600 SW Archer Road, PO Box 100424, Gainesville, Florida, 32610‐0424, USA
  2. 2 Department of Biochemistry and Molecular Biology, University of Calgary, 3330 Hospital Drive NW, Calgary, Alberta, T2N 4N1, Canada
  1. *Corresponding author. Tel: +1 352 392 6190; Fax: +1 352 392 4620; E‐mail: echan{at}ufl.edu
  1. These authors contributed equally to this work

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Abstract

GW bodies (GWBs), or mammalian P bodies, proposed to be involved in messenger RNA storage and/or degradation, have recently been linked to RNA interference and microRNA (miRNA) processing. We report that endogenous let‐7 miRNA co‐precipitates with the GW182 protein complex. In addition, knockdown of two proteins, Drosha and its protein partner DGCR8, which are vital to the generation of mature miRNA, results in the loss of GWBs. Subsequent introduction of short interference RNA specific to lamin A/C is accompanied by reassembly of GWBs and concurrent knockdown of lamin A/C protein. Taken together, these studies show that miRNAs are crucial components in GWB formation.

  • Received January 24, 2006.
  • Revision received July 12, 2006.
  • Accepted July 12, 2006.
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