Skip to main content
Advertisement
  • Other Publications
    • EMBO Press
    • EMBO reports (Home)
    • The EMBO Journal
    • EMBO Molecular Medicine
    • Molecular Systems Biology
    • Life Science Alliance
Login

   

Search

Advanced Search

Journal

  • Home
  • Latest Online
  • Current Issue
  • Archive
  • Subject Collections
  • Review Series & Focuses

Authors & Referees

  • Submit
  • Author Guidelines
  • Aims & Scope
  • Editors & Board
  • Transparent Process
  • Bibliometrics
  • Referee Guidelines
  • Open Access

Info

  • E-Mail Editorial Office
  • Alerts
  • RSS Feeds
  • Subscriptions & Access
  • Reprints & Permissions
  • Advertise & Sponsor
  • Media Partners
  • News & Press
  • Recommend to Librarian
  • Customer Service
  • Home
  • EMBO reports: 18 (8)

Transparent Process

Article

The structure of the tetanus toxin reveals pH‐mediated domain dynamics

View ORCID ProfileGeoffrey Masuyer, View ORCID ProfileJulian Conrad, View ORCID ProfilePål Stenmark
DOI 10.15252/embr.201744198 | Published online 23.06.2017
EMBO reports (2017) 18, 1306-1317
Geoffrey Masuyer
Department of Biochemistry and Biophysics, Stockholm University, Stockholm, Sweden
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Julian Conrad
Department of Biochemistry and Biophysics, Stockholm University, Stockholm, SwedenScience for Life Laboratory, Stockholm University, Solna, Sweden
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Pål Stenmark
Department of Biochemistry and Biophysics, Stockholm University, Stockholm, Sweden
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site

Author Affiliations

  1. Geoffrey Masuyer1,
  2. Julian Conrad1,2 and
  3. Pål Stenmark (stenmark{at}dbb.su.se)*,1
  1. 1Department of Biochemistry and Biophysics, Stockholm University, Stockholm, Sweden
  2. 2Science for Life Laboratory, Stockholm University, Solna, Sweden
  1. ↵*Corresponding author. Tel: +46 8163729; E‐mail: stenmark{at}dbb.su.se
View Abstract
  • Article
  • Figures & Data
  • Transparent Process
Loading

Abstract

The tetanus neurotoxin (TeNT) is a highly potent toxin produced by Clostridium tetani that inhibits neurotransmission of inhibitory interneurons, causing spastic paralysis in the tetanus disease. TeNT differs from the other clostridial neurotoxins by its unique ability to target the central nervous system by retrograde axonal transport. The crystal structure of the tetanus toxin reveals a “closed” domain arrangement stabilised by two disulphide bridges, and the molecular details of the toxin's interaction with its polysaccharide receptor. An integrative analysis combining X‐ray crystallography, solution scattering and single particle electron cryo‐microscopy reveals pH‐mediated domain rearrangements that may give TeNT the ability to adapt to the multiple environments encountered during intoxication, and facilitate binding to distinct receptors.

See also: M Montal (August 2017)

Synopsis

Embedded Image

Tetanus toxin is a deadly neurotoxin targeting the central nervous system. The structure of the holotoxin reveals pH‐mediated domain rearrangements important for sorting, stability, receptor‐interactions and the response to the environments encountered during intoxication.

  • The crystal structure of the tetanus holotoxin reveals a “closed” domain arrangement.

  • Single particle cryo‐EM presents a “semi‐open” domain organization.

  • pH regulates the positions of the tetanus toxin's binding domain.

  • clostridial toxin
  • tentoxilysin
  • tetanospasmin
  • tetanus neurotoxin

EMBO Reports (2017) 18: 1306–1317

  • Received March 8, 2017.
  • Revision received May 16, 2017.
  • Accepted May 23, 2017.
  • © 2017 The Authors
View Full Text

Subscribers, please sign in with your username and password.

Log in using your username and password

Enter your EMBO Reports username.
Enter the password that accompanies your username.
Forgot your user name or password?

Log in through your institution

You may be able to gain access using your login credentials for your institution. Contact your library if you do not have a username and password.
If your organization uses OpenAthens, you can log in using your OpenAthens username and password. To check if your institution is supported, please see this list. Contact your library for more details.

Pay Per Article - You may access this article (from the computer you are currently using) for 1 day for US$35.00

Regain Access - You can regain access to a recent Pay per Article purchase if your access period has not yet expired.

EMBO Members please login here to access the journals

Subscribe to the Journal

EMBO Journal

EMBO Reports

Recommend to your Librarian

EMBO Journal

EMBO Reports

 

 

Previous Article in this IssueNext Article in this Issue
Back to top

  • PDF
  • Share
  • Export
  • Print
Loading

PDF

Review Process

In this Issue
Volume 18, Issue 8
01 August 2017 | pp 1265 - 1472
EMBO reports: 18 (8)
About the cover
Alert me when this article is cited
Alert me if a correction is posted

Article

  • Article
    • Abstract
    • Synopsis
    • Introduction
    • Results
    • Discussion
    • Materials and Methods
    • Author contributions
    • Conflict of interest
    • Expanded View
    • Acknowledgements
    • References
  • Figures & Data
  • Transparent Process

Related Content

More Articles

  • The heptad repeat domain 1 of Mitofusin has membrane destabilization function in mitochondrial fusion
    Frédéric Daste, Cécile Sauvanet, Andrej Bavdek, James Baye, Fabienne Pierre, Rémi Le Borgne, Claudine David, Manuel Rojo, Patrick Fuchs, David Tareste
    EMBO reports : e43637
  • RNA sensor LGP2 inhibits TRAF ubiquitin ligase to negatively regulate innate immune signaling
    Jean‐Patrick Parisien, Jessica J Lenoir, Roli Mandhana, Kenny R Rodriguez, Kenin Qian, Annie M Bruns, Curt M Horvath
    EMBO reports : e45176
  • EphrinB2/EphB4 signaling regulates non‐sprouting angiogenesis by VEGF
    Elena Groppa, Sime Brkic, Andrea Uccelli, Galina Wirth, Petra Korpisalo‐Pirinen, Maria Filippova, Boris Dasen, Veronica Sacchi, Manuele Giuseppe Muraro, Marianna Trani, Silvia Reginato, Roberto Gianni‐Barrera, Seppo Ylä‐Herttuala, Andrea Banfi
    EMBO reports : e45054
More Article

Related Articles

Cited By...

Request Permissions

Subject Areas

  • Microbiology, Virology & Host Pathogen Interaction
  • Structural Biology

Journal

  • Latest Online
  • Current Issue
  • Archive
  • Bibliometrics
  • E-Mail Editorial Office

Authors & References

  • Aims & Scope
  • Editors & Board
  • Transparent Process
  • Author Guidelines
  • Referee Guidelines
  • Open Access
  • Submit

Info

  • Alerts
  • RSS Feeds
  • Subscriptions & Access
  • Reprints & Permissions
  • Advertise & Sponsor
  • News & Press
  • Recommend to Librarian
  • Customer Service

EMBO

  • Funding & Awards
  • Events
  • Science Policy
  • Members
  • About EMBO

Online ISSN  1469-3178

Copyright© 2018 EMBO

This website is best viewed using the latest versions of all modern web browsers. Older browsers may not display correctly.