Cover: Conservative DNA replication of telomeres in human cells that rely on alternative lengthening of telomeres (ALT) to escape replicative senescence. Telomere strand-specific fluorescence in situ hybridization (FISH), performed in three consecutive steps (denaturing FISH/non-denaturing chromosome orientation FISH/denaturing FISH), distinguishes between semiconservative and conservative telomeric replication. Images from the second (left) and third (right) staining steps show a chromosome (circled) with a telomeric end that is conservatively replicated (green, G-rich telomeric repeats; red, C-rich telomeric repeats; blue, DAPI). From Fani-Marlen Roumelioti, Sotirios K Sotiriou, Thanos D Halazonetis, Sarantis Gagos and colleagues: Alternative lengthening of human telomeres is a conservative DNA replication process with features of break-induced replication. For detail, see Scientific Report on page 1731.